Reporter

Part:BBa_K4799606

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pNtCCX2_GUS_T-Nos

pNtCCX2 is a promoter. It is added before the reporter gene β-Glucuronidase (gus) and NOS terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1910
    Illegal SpeI site found at 2207
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1910
    Illegal NheI site found at 4016
    Illegal SpeI site found at 2207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1910
    Illegal BglII site found at 1998
    Illegal BamHI site found at 1981
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1910
    Illegal SpeI site found at 2207
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1910
    Illegal SpeI site found at 2207
    Illegal NgoMIV site found at 2431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2231
    Illegal BsaI.rc site found at 2846
    Illegal SapI.rc site found at 3017


[edit]
Categories
Parameters
None